|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human hemoglobin.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-10 µg/ml|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-10808 detects Hemoglobin from human samples. This antibody does not detect bovine, chicken, equine, ovine, or porcine Hemoglobin.
MA1-10808 has been successfully used in ELISA and Western blot applications.
The MA1-10808 immunogen is purified human hemoglobin.
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.