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|Tested species reactivity||Virus|
|Published species reactivity||Virus, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Baculovirus expressed recombinant HBx.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-081 detects hepatitis B x (HBx) protein from transfected human cells.
MA1-081 has been successfully used in Western blot, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects a single ~17 kDa protein representing HBx. This antibody is not capable of immunoprecipitating HBx complexed with HBx interacting protein (XIP).
The MA1-081 immunogen is baculovirus expressed recombinant HBx.
The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries.
Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis B virus (HBV) induces the expression of interleukin-8 that in turn reduces HBV sensitivity to interferon-alpha.
MA1-081 was used in western blot to study the mechanism by which HBV infection promotes the IL-8 production that reduces the sensitivity of the virus to IFN-alpha
|Pollicino T,Bellinghieri L,Restuccia A,Raffa G,Musolino C,Alibrandi A,Teti D,Raimondo G||Virology (444:317)||2013|
The hepatitis B virus X protein functionally interacts with CREB-binding protein/p300 in the regulation of CREB-mediated transcription.
MA1-081 was used in western blot to study the role of the hepatitis B virus X protein in the regulation of CREB-mediated transcription
|Cougot D,Wu Y,Cairo S,Caramel J,Renard CA,Lévy L,Buendia MA,Neuveut C||The Journal of biological chemistry (282:4277)||2007|
Calpain-specific proteolysis in primate retina: Contribution of calpains in cell death.
MA1-081 was used in western blot to study the involvement of calpain-induced proteolysis in retinal cell death in primates
|Nakajima E,David LL,Bystrom C,Shearer TR,Azuma M||Investigative ophthalmology & visual science (47:5469)||2006|
State of hepatitis C viral replication enhances activation of NF-kB- and AP-1-signaling induced by hepatitis B virus X.
MA1-081 was used in western blot to study the possible involvement of hepatitis B virus X in HCV-related hepatocarcinogenesis
|Kanda T,Yokosuka O,Nagao K,Saisho H||Cancer letters (234:143)||2006|
HBx or HCV core gene expression in HepG2 human liver cells results in a survival benefit against oxidative stress with possible implications for HCC development.
MA1-081 was used in immunocytochemistry to study the protective effect of HBx or HCV core gene expression against oxidative stress in HepG2 cells
|Severi T,Vander Borght S,Libbrecht L,VanAelst L,Nevens F,Roskams T,Cassiman D,Fevery J,Verslype C,van Pelt JF||Chemico-biological interactions (168:128)||2007|
Detection of hepatitis C virus RNA sequences in B-cell non-Hodgkin lymphoma.
MA1-081 was used in immunohistochemistry to investigate the role of hepatitis C virus in the pathogenesis of B-cell non-Hodgkin lymphomas
|Karavattathayyil SJ,Kalkeri G,Liu HJ,Gaglio P,Garry RF,Krause JR,Dash S||American journal of clinical pathology (113:391)||2000|
Cloning and characterization of a novel hepatitis B virus x binding protein that inhibits viral replication.
MA1-081 was used in immunoprecipitation to identify and characterize a new hepatitis B virus x binding protein.
|Melegari M,Scaglioni PP,Wands JR||Journal of virology (72:1737)||1998|