|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1/100|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
|Western Blot (WB)||See 44 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 57 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|ELISA (ELISA)||See 2 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Tested Species reactivity||Virus|
|Published species reactivity||Virus , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified HCV core-GST fusion protein (genotype 1b).|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-080 detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
MA1-080 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. By Western blot, this antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines. Immunofluorescence staining of HCV core protein in transfected chimp hepatocytes yields a staining pattern consistent with cytoplasmic and vesicular staining.
The MA1-080 immunogen is purified HCV core-GST fusion protein (genotype 1b). This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HCV core; HepC; HepC core
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