Western blot staining of HEK293 lysate overexpressing Human MYF6 (Myc tagged and loaded in Lane A and C) using PA5-19291 at a concentration of 0.5ug/ml (Lane A). Lane C was stained with anti-MYC Tag at a dilution of 1/1000. Lane B was loaded with mock-transfected HEK293 cells and stained using PA5-19291 at a concentration of 0.5 mg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (SYRPKQENLEGAD) corresponding to the internal amino acids of MYF6|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.
The protein encoded by this gene is a probable basic helix-loop-helix DNA binding protein involved in muscle differentiation. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of autosomal dominant centronuclear myopathy .
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