|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog , Rat , Human Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant extracellular domain of rat NDF protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-12893 targets Heregulin in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
The MA5-12893 immunogen is recombinant extracellular domain of rat NDF protein.
Heregulin (HRG) or neu differentiation factor (NDF) binds to c-erbB-3 and c-erbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, alpha and beta that differ in their EGF-like domain and in receptor binding affinity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: acetylcholine receptor-inducing activity; alpha (45kD; ERBB2 p185-activator); glial growth factor; glial growth factor 2; heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); membrane-bound isoform; neu differentiation factor; neuregulin 1 type III beta 3; pro-neuregulin-1; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; sensory and motor neuron derived factor; type I neuregulin 1; type III neuregulin 1
Gene Aliases: 6030402G23Rik; ARIA; D230005F13Rik; GGF; GGF2; GGFII; HGL; HRG; HRG1; HRGA; HRGalpha; MST131; MSTP131; NDF; NRG1; NRG1-IT2; SMDF
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