Immunofluorescence analysis of NRG1 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NRG1 (7D5) Mouse Monoclonal Antibody (MA512896) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous, cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Non-human primate, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant extracellular domain of rat NDF protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12896 targets Heregulin in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12896 immunogen is recombinant extracellular domain of rat NDF protein.
Heregulin (HRG) or neu differentiation factor (NDF) binds to c-erbB-3 and c-erbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, alpha and beta that differ in their EGF-like domain and in receptor binding affinity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model.
MA5-12896 was used in western blot to study the alterations of brain Neuregulin-1 signaling during neuroinflammation
|Yang Z,Jiang Q,Chen SX,Hu CL,Shen HF,Huang PZ,Xu JP,Mei JP,Zhang BP,Zhao WJ||Molecular medicine reports (14:790)||2016|
Neuregulin-1-human epidermal receptor-2 signaling is a central regulator of pulmonary epithelial permeability and acute lung injury.
MA5-12896 was used in western blot to study the role of neuregulin-1 and HER2 in regulating pulmonary epithelial permeability and acute lung injury
|Finigan JH,Faress JA,Wilkinson E,Mishra RS,Nethery DE,Wyler D,Shatat M,Ware LB,Matthay MA,Mason R,Silver RF,Kern JA||The Journal of biological chemistry (286:10660)||2011|
Phenotypic characterization of transgenic mice overexpressing neuregulin-1.
MA5-12896 was used in western blot to study the cognitive and behavioural phenotype of transgenic mice overexpressing neuregulin-1
|Kato T,Kasai A,Mizuno M,Fengyi L,Shintani N,Maeda S,Yokoyama M,Ozaki M,Nawa H||PloS one (5:null)||2010|
Neuregulin-1 signaling in brain endothelial cells.
MA5-12896 was used in western blot to study neuregulin-1 signaling in brain microvascular endothelial cells
|Lok J,Sardi SP,Guo S,Besancon E,Ha DM,Rosell A,Kim WJ,Corfas G,Lo EH||Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (29:39)||2009|
Neuregulin-1 enhances survival of human astrocytic glioma cells.
MA5-12896 was used in western blot to study the mechanism by which neuregulin-1 promotes the survival of human astrocytoma cells
|Ritch PS,Carroll SL,Sontheimer H||Glia (51:217)||2005|
Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey.
MA5-12896 was used in immunohistochemistry to study the expression of neuregulin-1 and its receptors in various Rhesus monkey endocrine tissues
|Zhao WJ||International journal of endocrinology and metabolism (11:162)||2013|
Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels.
MA5-12896 was used in immunohistochemistry to investigate the extracellular and protein abnormalities in peripheral neuropathy
|Kagitani-Shimono K,Mohri I,Yagi T,Taniike M,Suzuki K||Acta neuropathologica (115:577)||2008|
Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain.
MA5-12896 was used in immunohistochemistry to study the localization of neuregulin-1alpha and ErbB-4 tyrosine kinase in developing and adult human brain
|Bernstein HG,Lendeckel U,Bertram I,Bukowska A,Kanakis D,Dobrowolny H,Stauch R,Krell D,Mawrin C,Budinger E,Keilhoff G,Bogerts B||Brain research bulletin (69:546)||2006|
NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.
MA5-12896 was used in immunohistochemistry to investigate the association of NRG1 gene rearrangements with poor prognosis in breast cancer treatment
|Prentice LM,Shadeo A,Lestou VS,Miller MA,deLeeuw RJ,Makretsov N,Turbin D,Brown LA,Macpherson N,Yorida E,Cheang MC,Bentley J,Chia S,Nielsen TO,Gilks CB,Lam W,Huntsman DG||Oncogene (24:7281)||2005|
Expression of heregulin in human coronary atherosclerotic lesions.
MA5-12896 was used in immunohistochemistry to study the expression of heregulin in human coronary atherosclerotic lesions
|Panutsopulos D,Arvanitis DL,Tsatsanis C,Papalambros E,Sigala F,Spandidos DA||Journal of vascular research (42:463)||2005|
Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group.
MA5-12896 was used in immunohistochemistry to investigate the efficacy of the combination of paclitaxel, carboplatin and gefitinib against advanced breast cancer
|Fountzilas G,Pectasides D,Kalogera-Fountzila A,Skarlos D,Kalofonos HP,Papadimitriou C,Bafaloukos D,Lambropoulos S,Papadopoulos S,Kourea H,Markopoulos C,Linardou H,Mavroudis D,Briasoulis E,Pavlidis N,Razis E,Kosmidis P,Gogas H||Breast cancer research and treatment (92:1)||2005|
Role of neuregulin-1/ErbB2 signaling in endothelium-cardiomyocyte cross-talk.
MA5-12896 was used in immunoprecipitation to study the role of neuregulin-1/ErbB2 signaling in endothelium-cardiomyocyte cross-talk
|Lemmens K,Segers VF,Demolder M,De Keulenaer GW||The Journal of biological chemistry (281:19469)||2006|
Constitutive neuregulin-1/ErbB signaling contributes to human vestibular schwannoma proliferation.
MA5-12896 was used in immunoprecipitation and western blot to study the role of constitutive neuregulin-1/ErbB signaling in human vestibular schwannoma proliferation
|Hansen MR,Roehm PC,Chatterjee P,Green SH||Glia (53:593)||2006|
Back signaling by the Nrg-1 intracellular domain.
MA5-12896 was used in immunocytochemistry to study the bidirectional signaling of neuregulin-1 in neurones and the role of the intracellular domain in retrograde signaling
|Bao J,Wolpowitz D,Role LW,Talmage DA||The Journal of cell biology (161:1133)||2003|
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
MA5-12896 was used in immunocytochemistry to study the role of neuregulin and neurotrophins in regulating spiral ganglion neuron-Schwann cell reciprocal signaling in the auditory nerve
|Hansen MR,Vijapurkar U,Koland JG,Green SH||Hearing research (161:87)||2001|