Immunofluorescent analysis of Hexokinase II showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a Hexokinase II polyclonal antibody (Product # PA5-29326) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Horse, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 53 and 342 of Human Hexokinase II|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29326 targets Hexokinase II in WB, IF and IHC(P) applications and shows reactivity with Human, mouse and horse samples. Strong reactivity with Hexokinase 2 was observed in Western Blots using horse endometrial samples and mouse brain lysates. No reactivity in WB could be observed using mouse endometrial whole cell lysates.
The PA5-29326 immunogen is recombinant fragment corresponding to a region within amino acids 53 and 342 of Human Hexokinase II.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.