Western blot of Hic-1 in (A) A549 and (B) Jurkat lysates was performed using 35ug protein in RIPA buffer. Lysates were probed with a Hic-1 polyclonal antibody (Product# PA5-37865) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence HLEGYPCPHLAYGEP, from the internal region of HIC1 (aa396-410)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog, pig and cow based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.