|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified high molecular weight kininogen.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-10 µg/ml|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-10832 detects High MW Kininogen, heavy chain, from human samples.
MA1-10832 has been successfully used in ELISA and Western blot applications.
The MA1-10832 immunogen is purified high molecular weight kininogen.
This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
MA1-10832 was used in ELISA and western blot to investigate the specificity of interleukin 12 antibodies in vitro
|Wilkinson VL,Warrier RR,Truitt TP,Nunes P,Gately MK,Presky DH||Journal of immunological methods (189:15)||1996|