Western blot analysis of Histamine H4 Receptor using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate and C) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Histamine H4 Receptor polyclonal antibody (Product # PA5-21313) at a dilution of 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 82 of Histamine H4 Receptor (Uniprot ID#Q9H3N8)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21313 targets Histamine H4 Receptor in WB applications and shows reactivity with Human samples.
The PA5-21313 immunogen is a synthetic peptide corresponding to a region within amino acids 1 and 82 of Histamine H4 Receptor (Uniprot ID#Q9H3N8).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.