Western blot analysis of Histone 2A in HeLa (lane 1) NIH-3T3 (lane 2) and COS7 cell lysate (lane 3) (30ug/each lane) using a Histone 2A recombinant rabbit monoclonal antibody (Product # 700158) at a dilution of 1ug/ml. Samples were blocked in 5% milk/TBST and detection was performed using an AP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:10,000. NBT/BCIP was used as the substrate (Product # WB7105).
|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 2-19 of human Histone 2A.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Histones are nuclear proteins that play an important role in maintaining the structure of the chromosome in eukaryotes. The nucleosome is made up of around 146bp of DNA, which enfolds around a histone octomer unit comprising of four major histones, H2A, H2B, H3 and H4. An important role of H2A is to ensure proper assembly or functioning of the centrometre. They also play an important role in gene regulation. The protein can be modified by acetylation and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.