|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Nuclei from myeloid leukemia biopsy cells.|
|Storage buffer||PBS, pH 7.2, with 40% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||5-20 µg/ml|
|Immunofluorescence (IF)||5-20 ug/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91853 detects Histone H1 from human samples.
MA1-91853 has been successfully used in ELISA, immunocytochemistry, and Western blot applications.
The MA1-91853 immunogen is Nuclei from myeloid leukemia biopsy cells.
Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.