Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Histone H1 (green) showing staining in the nucleus of human tonsil tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a Histone H1 monoclonal antibody (Product # MA5-13750) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Nuclei of myeloid leukemia biopsy cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13750 targets Histone H1 in IF, WB and IHC (P) applications and shows reactivity with human and mouse samples.
The MA5-13750 immunogen is nuclei of myeloid leukemia biopsy cells.
This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bcl-2 sustains hormetic response by inducing Nrf-2 nuclear translocation in L929 mouse fibroblasts.
MA5-13750 was used in western blot to investigate the mechanism of hormesis in L929 cell lines
|Luna-López A,Triana-Martínez F,López-Diazguerrero NE,Ventura-Gallegos JL,Gutiérrez-Ruiz MC,Damián-Matsumura P,Zentella A,Gómez-Quiroz LE,Königsberg M||Free radical biology and medicine (49:1192)||2010|
Histone H1 is released from myonuclei and present in rimmed vacuoles with DNA in inclusion body myositis.
MA5-13750 was used in immunohistochemistry to investigate the release of histone H1 into rimmed vacuoles in inclusion body myositis
|Nakano S,Shinde A,Fujita K,Ito H,Kusaka H||Neuromuscular disorders : NMD (18:27)||2008|
Spermatid-specific linker histone H1-like protein; TISP64
H1.9; Hils1; TISP64