|Tested species reactivity||Chicken, Human, Mouse, Non-human primate, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide designed based on the region around pThreonine 77 of human Histone H2A sequence|
|Purification||Antigen affinity chromatography, Protein A|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous levels of total histone H2A protein. This antibody also detects histone H2A.X and histone H2A.Z.
It is not recommended to aliquot this antibody.
Histones are nuclear proteins that play an important role in maintaining the structure of the chromosome in eukaryotes. The nucleosome is made up of around 146bp of DNA, which enfolds around a histone octomer unit comprising of four major histones, H2A, H2B, H3 and H4. An important role of H2A is to ensure proper assembly or functioning of the centrometre. They also play an important role in gene regulation. The protein can be modified by acetylation and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.