Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human small intestine stained using a Histone H3.3 polyclonal antibody (Product # PA5-23288) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Bovine, Chicken, Fruit fly, Horse, Human, Marsupial, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (119 TIMPKDIQLARRIRGERA 136) of Histone H3.3 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.1-1 µg/ml|
|Western Blot (WB)||0.25-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone H3.3 is a nuclear protein and a replication-independent member of the histone H3 family that has a role in nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Expression of H3F3A is common throughout the cell cycle independent of DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.