Immunofluorescent analysis of Histone H3.3B showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Histone H3.3B polyclonal antibody (Product # PA5-22388) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 (P84243) of Histone H3.3B|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22388 targets Histone H3.3B in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, Rat and mouse samples.
The PA5-22388 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 (P84243) of Histone H3.3B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.