HeLa cells were fixed with methanol and blocked with PBS containing 2.5% BSA. (A) Cells were labeled with the anti-H3K36me1 antibody (diluted 1:200 and incubated for 1 hour at room temperature) with a labeled goat anti-rabbit secondary antibody). (B) Nuclei were stained using the DNA-specific stain DAPI. Both the anti-H3K36me1 antibody and DAPI produced clear nuclear staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the region of the histone H3 containing the monomethylated lysine 36 (H3K36me1), using a KLH-conjugated synthetic peptide.|
|Contains||0.05% sodium azide, 0.05% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5 µg|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.