Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H4K8ac crude serum (Cat. no. 49-1022), diluted 1:250 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right. Lane 2 shows the result of the western analysis with the crude serum; lane 1 shows the same analysis after incubation of the crude serum with 750 pmol blocking peptide for 1 hour at room temperature.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against histone H4 containing the acetylated lysine 8 (H4K8ac), using a KLH-conjugated synthetic peptide.|
|Storage buffer||whole serum|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5 ug|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
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