|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein between amimo acids 699-908 of the rat HSL protein.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-16966 is specific for the ~84 kDa Hormone-Sensitive Lipase protein.
A non-specific band may also be detected at ~89 kDa.
Bands at ~102, 113, and 127 kDa may also be seen in the testis of sexually immature rats.Suggested positive control: human adipose protein.
Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides and cholesteryl esters. It is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATGL and HSL are not coordinately regulated in response to fuel partitioning in fasted rats.
PA1-16966 was used in western blot to study the regulation of adipose triglyceride lipase and hormone sensitive lipase during prolonged starvation.
|Bertile F,Raclot T||The Journal of nutritional biochemistry (22:372)||2011|