|Tested species reactivity||Human, Mouse, Pig|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 341 and 565 of Human Hsc70|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-29241 targets Hsc70 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-29241 immunogen is recombinant fragment corresponding to a region within amino acids 341 and 565 of Human Hsc70.
The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial proteome remodeling in ischemic heart failure.
PA5-29241 was used in western blot to study the effects of ischemic heart failure on the mitochondrial proteome
|Liu T,Chen L,Kim E,Tran D,Phinney BS,Knowlton AA||Life sciences (101:27)||2014|