|Tested species reactivity||Human|
|Host / Isotype||Insect|
|Storage buffer||TBS, pH 7.5, with 0.05% Brij-35, 5mM CaCl2, 50mM Imidazole|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-77538 is purified recombinant human HtrA1 protein (C-terminal His tagged), expressed in insect cells.
RP-77538 may be used in inhibition assays and as a control with corresponding anti-HtrA1 antibodies.
RP-77538 appears as a major protein of about 53 kDa in SDS-PAGE.
Secreted human HtrA1 serine protease is supposed to form oligomers of identical protein chains as revea;ed by X-ray analysis for the bacterial HtrA protein DegP. Polypeptide chains of human HtrA1 consist of several domains: An N-terminal insulin-like growth factor domain is followed by a Kazal-type serine protease inhibitor domain, a linker region, a trypsin-like protease domain and a PDZ domain. The function of HtrA1 appears closely linked to signaling by proteins of the TGFbeta family. During mouse embryo development HtrA1 is localized in specific areas where signaling by TGFbeta family proteins occurs. HtrA1 binds TGFbeta, BMP4, Gdf5 and activan. It inhibits signaling by these factors. For inhibition serine protease activity of HtrA1 is essentia. HtrA1 is upregulated in osteoarthritic cartilage. Increased cartilage HtrA1 may possibly aggravate osteoarthritis by antagonizing TGFbeta and thereby promoting terminal differentiation of articular chondrocyte. In later stages of tumor progression HtrA1 in down regulated. As a protease HtrA1 hydrolizes type II procllagen alpha1 C-propeptide, decorin and biglycan. Proteolytic activity of HtrA1 is regulated by ligands binding to PDZ domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.