Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Cat. no. S11226). Both sections were counterstained with DAPI (Cat. no. D1306, D3571, D21490) (Cat. No. A21272)
|Tested species reactivity||Human|
|Published species reactivity||Rat, Pig, Zebrafish, Mouse, Human, Not Applicable, Guinea pig|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Human HuC/HuD neuronal protein.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 10 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 8 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sirtuin-3 Is Expressed by Enteric Neurons but It Does not Play a Major Role in Their Regulation of Oxidative Stress.
A-21272 was used in immunohistochemistry to determine how enteric neurons express sirtuin-3 but do not play a major role in the regulation of oxidative stress
|Bubenheimer RK,Brown IA,Fried DE,McClain JL,Gulbransen BD||Frontiers in cellular neuroscience (10:null)||2016|
Enteric glia mediate neuron death in colitis through purinergic pathways that require connexin-43 and nitric oxide.
A-21272 was used in immunohistochemistry to analyze the requirement of connexin-43 and nitric oxide for purinergic pathways that enteric glia mediate in neuron death in colitis
|Brown IA,McClain JL,Watson RE,Patel BA,Gulbransen BD||Cellular and molecular gastroenterology and hepatology (2:77)||2016|
Hepatocyte Growth Factor and MET Support Mouse Enteric Nervous System Development, the Peristaltic Response, and Intestinal Epithelial Proliferation in Response to Injury.
A-21272 was used in immunohistochemistry to research response to injury and the role of hepatocyte growth factor and peristaltic response, MET support mouse enteric nervous system development, and intestinal epithelial proliferation
|Avetisyan M,Wang H,Schill EM,Bery S,Grider JR,Hassell JA,Stappenbeck T,Heuckeroth RO||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:11543)||2015|
Enteric neuropathy can be induced by high fat diet in vivo and palmitic acid exposure in vitro.
A-21272 was used in immunohistochemistry to study lipid-induced intestinal changes
|Voss U,Sand E,Olde B,Ekblad E||PloS one (8:null)||2013|
Enteric neuropathy evoked by repeated cisplatin in the rat.
A-21272 was used in immunohistochemistry to assess the prolonged effects of repeated cisplatin administration on the rat enteric nervous system.
|Vera G,Castillo M,Cabezos PA,Chiarlone A,Martín MI,Gori A,Pasquinelli G,Barbara G,Stanghellini V,Corinaldesi R,De Giorgio R,Abalo R||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (23:370)||2011|
Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E.
A-21272 was used in immunohistochemistry to study the expression of serotonin receptor subtypes in the human colon.
|Kapeller J,Möller D,Lasitschka F,Autschbach F,Hovius R,Rappold G,Brüss M,Gershon MD,Niesler B||The Journal of comparative neurology (519:420)||2011|
Protein kinase C modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion.
A-21272 was used in immunohistochemistry to study how ischemic episodes alter glutamate levels and activation of NMDA in the gastrointestinal tract.
|Giaroni C,Zanetti E,Giuliani D,Oldrini R,Marchet S,Moro E,Borroni P,Trinchera M,Crema F,Lecchini S,Frigo G||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (23:e91)||2011|
Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration.
A-21272 was used in immunohistochemistry to investigate CNS regeneration in zebrafish
|Ramachandran R,Reifler A,Parent JM,Goldman D||The Journal of comparative neurology (518:4196)||2010|
Postnatal maturation of the gastrointestinal tract: a functional and immunohistochemical study in the guinea-pig ileum at weaning.
A-21272 was used in immunohistochemistry to examine the longitudinal muscle-myenteric plexus in weaning guinea pigs
|Abalo R,Vera G,Rivera AJ,Moro-Rodríguez E,Martín-Fontelles MI||Neuroscience letters (467:105)||2009|
Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody.
A-21272 was used in immunohistochemistry to quantitate glial cells in the human and guinea pig enteric nervous system.
|Hoff S,Zeller F,von Weyhern CW,Wegner M,Schemann M,Michel K,Rühl A||The Journal of comparative neurology (509:356)||2008|
Mechanosensitive enteric neurons in the guinea pig gastric corpus.
A-21272 was used in immunocytochemistry to elucidate the guinea pig gastric corpus and mechanosensitive enteric neurons
|Mazzuoli-Weber G,Schemann M||Frontiers in cellular neuroscience (9:null)||2015|
Lipopolysaccharide-induced loss of cultured rat myenteric neurons - role of AMP-activated protein kinase.
A-21272 was used in immunocytochemistry to study the role of AMP-activated protein kinase in the loss of cultured rat myenteric neurons due to lipopolysaccharide-induced loss
|Voss U,Ekblad E||PloS one (9:null)||2014|
The enteric nervous system of P2Y13 receptor null mice is resistant against high-fat-diet- and palmitic-acid-induced neuronal loss.
A-21272 was used in immunocytochemistry to study the role of the ADP-sensitive P2Y3 receptor in lipid-induced enteric neuropathy.
|Voss U,Turesson MF,Robaye B,Boeynaems JM,Olde B,Erlinge D,Ekblad E||Purinergic signalling (10:455)||2014|
5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice.
A-21272 was used in immunocytochemistry to test if the 5-HT(4) receptor promotes enteric neuron survival and neurogenesis
|Liu MT,Kuan YH,Wang J,Hen R,Gershon MD||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:9683)||2009|
Hyperplasia of interstitial cells of cajal in sprouty homolog 4 deficient mice.
A-21272 was used in immunohistochemistry - frozen section to study sprouty homolog 4 deficient mice and hyperplasia of interstitial cells of cajal
|Thys A,Vandenberghe P,Hague P,Klein OD,Erneux C,Vanderwinden JM||PloS one (10:null)||2015|
Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling.
A-21272 was used in immunohistochemistry - frozen section to elucidate the function of bone morphogenetic proteins in enteric gliogenesis.
|Chalazonitis A,D'Autréaux F,Pham TD,Kessler JA,Gershon MD||Developmental biology (350:64)||2011|
Natural compounds boldine and menthol are antagonists of human 5-HT3 receptors: implications for treating gastrointestinal disorders.
A-21272 was used in immunohistochemistry to study the effect of 5-HT3 receptor modulation on disease.
|Walstab J,Wohlfarth C,Hovius R,Schmitteckert S,Röth R,Lasitschka F,Wink M,Bönisch H,Niesler B||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (26:810)||2014|
Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.
A-21272 was used in immunohistochemistry to test if nongenetic factors might contribute to Hirschsprung disease using a zebrafish model.
|Lake JI,Tusheva OA,Graham BL,Heuckeroth RO||The Journal of clinical investigation (123:4875)||2013|
Retinaldehyde dehydrogenase enzymes regulate colon enteric nervous system structure and function.
A-21272 was used in immunohistochemistry to assess the role of retinoic acid metabolism in the development of the enteric nervous system.
|Wright-Jin EC,Grider JR,Duester G,Heuckeroth RO||Developmental biology (381:28)||2013|
Pax6 regulates gene expression in the vertebrate lens through miR-204.
A-21272 was used in immunohistochemistry (paraffin) to investigate the relationship between miR-204 and Pax6 in the eye.
|Shaham O,Gueta K,Mor E,Oren-Giladi P,Grinberg D,Xie Q,Cvekl A,Shomron N,Davis N,Keydar-Prizant M,Raviv S,Pasmanik-Chor M,Bell RE,Levy C,Avellino R,Banfi S,Conte I,Ashery-Padan R||PLoS genetics (9:null)||2013|
Regenerative process evaluation of neuronal subclasses in chagasic patients with megacolon.
A-21272 was used in immunohistochemistry to assess the regeneration tax of every neuronal cell type in the enteric nervous system from chagasic patients with megacolon and non-infected individuals.
|Moreira MD,Brehmer A,de Oliveira EC,Neto SG,Luquetti AO,Bueno LL,Fujiwara RT,de Freitas MA,da Silveira AB||Human immunology (74:181)||2013|
Neuronal serotonin regulates growth of the intestinal mucosa in mice.
A-21272 was used in immunohistochemistry (frozen) to test if serotonin affects growth and maintenance of the intestinal mucosa in mice.
|Gross ER,Gershon MD,Margolis KG,Gertsberg ZV,Li Z,Cowles RA||Gastroenterology (143:408)||2012|
Dual embryonic origin of the mammalian otic vesicle forming the inner ear.
A-21272 was used in immunohistochemistry to show that neuroepithelial cells contribute directly to the otic vesicle from the neural tube.
|Freyer L,Aggarwal V,Morrow BE||Development (Cambridge, England) (138:5403)||2011|
Differential regional and subtype-specific vulnerability of enteric neurons to mitochondrial dysfunction.
A-21272 was used in immunohistochemistry to determine the role of mitochondrial deficits in enteric neurodegeneration and gastrointestinal dysfunction.
|Viader A,Wright-Jin EC,Vohra BP,Heuckeroth RO,Milbrandt J||PloS one (6:null)||2011|
|Not Applicable||10 µg/ml||
Myenteric plexus injury and apoptosis in experimental colitis.
A-21272 was used in immunohistochemistry - paraffin section to assess the neural consequences of distal colitis and determine the role of neutrophil granulocytes in mediating these changes
|Boyer L,Ghoreishi M,Templeman V,Vallance BA,Buchan AM,Jevon G,Jacobson K||Autonomic neuroscience : basic and clinical (117:41)||2005|