Invitrogen

HuC/HuD Monoclonal Antibody (16A11), Biotin

Product Image Gallery

HuC/HuD Antibody in Immunohistochemistry (IHC) — Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Product # N-21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Product # S-11226). Both sections were counterstained with DAPI (Product # D1306, D3571, D21490) (Product # A-21272)

HuC/HuD Antibody in Immunocytochemistry (ICC/IF) — Fig. 2. The degree of selective neuronal hypoplasia is sustained as a function of age in the submucosal plexus of Cdnf -/- mice while in the myenteric plexus the age-dependent decline is similar with no hypoplasia compared to Cdnf +/+ mice; ( A ) Transcripts encoding CDNF are expressed in stomach, intestine and colon of the murine gut at P7 but are absent in Cdnf -/- mice. ( B ) Neuronal densities per mm 2 of plexus in the ileal submucosal plexuses, and (C) in the myenteric plexus in Cdnf -/- mice (magenta solid line) and Cdnf +/+ mice (blue solid line) are plotted as a function of age. The neuronal densities in the submucosal plexus are significantly lower in Cdnf -/- mice than in Cdnf +/+ mice at each age examined; [B] 2 way ANOVA F(2, 437) = 22.94. There is however some compensation between 1.5 and 11 month of age in Cdnf -/- mice which does not occur in the Cdnf +/+ mice; [C] in contrast in the myenteric plexus the decline as a function of age is similar in Cdnf -/- and in Cdnf +/+ mice without compensation between the genotypes. The numbers of animals were 3 Cdnf -/- and 3 Cdnf +/+ for 1.5 month-old mice; 6 Cdnf -/- and 6 Cdnf +/+ for 3 month-old animals; 7 Cdnf -/- and 7 Cdnf +/+ for 9-12 month-old mice; (D) Immunocytochemical detection of neurons using antibodies to HuC/D in whole mount laminar preparations of the ileal submucosal plexus in Cdnf +/+ (D 1 -D 3 ) and Cdnf -/- mice (D 4 -D 6 ) (scale bar, 32 mum).

HuC/HuD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using HuC/HuD monoclonal antibody (Product # A-21272) in Immunocytochemistry and Immunohistochemistry

HuC/HuD Antibody in In Situ Hybridization (ISH) (ISH) — Published figure using HuC/HuD monoclonal antibody (Product # A-21272) in In Situ Hybridization (ISH)

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

Immunohistochemistry (IHC)

5-20 µg/mL

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

ELISA (ELISA)

1-10 µg/mL

In Situ Hybridization (ISH) (ISH)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Guinea pig, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16A11

Immunogen

Human HuC/HuD neuronal protein.

Conjugate

Biotin

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2535822

Product Specific Information

This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.

Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.

For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.

Target Information

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HuC; HuD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21

Gene Aliases: ELAVL3; ELAVL4; HUC; HUCL; HUD; PLE21; PNEM

UniProt ID: (Human) Q14576, (Human) P26378

Entrez Gene ID: (Human) 1995, (Human) 1996

Molecular Function: translation initiation factor