Invitrogen

HuC/HuD Monoclonal Antibody (16A11), Biotin

Product Image Gallery

HuC/HuD Antibody in Immunofluorescence (IF) — Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Product # N-21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Product # S-11226). Both sections were counterstained with DAPI (Product # D1306, D3571, D21490) (Product # A-21272)

HuC/HuD Antibody — Published figure using HuC/HuD monoclonal antibody (Product # A-21272)

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1-10 µg/mL

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (IHC)

5-20 µg/mL

Western Blot (WB)

1-5 µg/mL

Published Applications

Immunofluorescence (IF)

See 4 publications below

Immunohistochemistry (IHC)

See 13 publications below

In Situ Hybridization (ISH) (ISH)

See 1 publication below

Immunocytochemistry (ICC)

See 5 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 2 publications below

Miscellaneous PubMed (MISC)

See 9 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Guinea pig, Human, Mouse, Pig, Rat, Zebrafish

Host / Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16A11

Immunogen

Human HuC/HuD neuronal protein.

Conjugate

Biotin

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2535822

Product Specific Information

This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.

Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.

For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.

Target Information

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; ELAVL3; ELAVL4; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HUC; HUD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; PLE21; PNEM

Gene Aliases: HUC; HUCL; HUD; PLE21; PNEM

Entrez Gene ID: (Human) 1995, (Human) 1996

Function(s)

RNA processing mRNA processing nervous system development cell differentiation

Process(es)

nucleotide binding AU-rich element binding RNA binding mRNA 3'-UTR binding