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Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Cat. no. S11226). Both sections were counterstained with DAPI (Cat. no. D1306, D3571, D21490) (Cat. No. A21272)
|Tested species reactivity||Human|
|Published species reactivity||Rat, Pig, Zebrafish, Mouse, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Human HuC/HuD neuronal protein.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The enteric nervous system of P2Y13 receptor null mice is resistant against high-fat-diet- and palmitic-acid-induced neuronal loss.
A-21272 was used in immunocytochemistry to study the role of the ADP-sensitive P2Y3 receptor in lipid-induced enteric neuropathy.
|Voss U,Turesson MF,Robaye B,Boeynaems JM,Olde B,Erlinge D,Ekblad E||Purinergic signalling (10:455)||2014|
Enteric neuropathy evoked by repeated cisplatin in the rat.
A-21272 was used in immunohistochemistry to assess the prolonged effects of repeated cisplatin administration on the rat enteric nervous system.
|Vera G,Castillo M,Cabezos PA,Chiarlone A,Martín MI,Gori A,Pasquinelli G,Barbara G,Stanghellini V,Corinaldesi R,De Giorgio R,Abalo R||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (23:370)||2011|
Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E.
A-21272 was used in immunohistochemistry to study the expression of serotonin receptor subtypes in the human colon.
|Kapeller J,Möller D,Lasitschka F,Autschbach F,Hovius R,Rappold G,Brüss M,Gershon MD,Niesler B||The Journal of comparative neurology (519:420)||2011|
Protein kinase C modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion.
A-21272 was used in immunohistochemistry to study how ischemic episodes alter glutamate levels and activation of NMDA in the gastrointestinal tract.
|Giaroni C,Zanetti E,Giuliani D,Oldrini R,Marchet S,Moro E,Borroni P,Trinchera M,Crema F,Lecchini S,Frigo G||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (23:e91)||2011|
Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration.
A-21272 was used in immunohistochemistry to investigate CNS regeneration in zebrafish
|Ramachandran R,Reifler A,Parent JM,Goldman D||The Journal of comparative neurology (518:4196)||2010|
Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody.
A-21272 was used in immunohistochemistry to quantitate glial cells in the human and guinea pig enteric nervous system.
|Hoff S,Zeller F,von Weyhern CW,Wegner M,Schemann M,Michel K,Rühl A||The Journal of comparative neurology (509:356)||2008|
Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling.
A-21272 was used in immunohistochemistry - frozen section to elucidate the function of bone morphogenetic proteins in enteric gliogenesis.
|Chalazonitis A,D'Autréaux F,Pham TD,Kessler JA,Gershon MD||Developmental biology (350:64)||2011|
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; ELAVL3; ELAVL4; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HUC; HUD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; PLE21; PNEM
ELAVL3; ELAVL4; HUC; HUCL; HUD; PLE21; PNEM