Invitrogen

HuR Monoclonal Antibody (3A2)

HuR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

HuR Antibody (MA1-167) in ICC/IF

Immunofluorescence analysis of HuR was performed using 70% confluent log phase HeLa cells treated with Actinomycin D (1uM for 19hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HuR Monoclonal Antibody (3A2) (Product # MA1-167) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Flu... View More

HuR Antibody in Immunoprecipitation (IP)

HuR Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

HuR Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using HuR Monoclonal Antibody (3A2) (Product # MA1-167), shows translocation from nucleus to cytoplasm in HeLa cell line when treated with Actinomycin D. {TM}

Product Details

MA1-167

Applications

Tested Dilution

Publications

Western Blot (WB)

1:125-1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:25-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/10^6 cells

RNA Immunoprecipitation (RIP)

2 µg/10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A2

Immunogen

Recombinant human HuR protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536870

Target Information

The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of human antigen R (HuR) in modulating proliferation, senescence and radiosensitivity of skin cells.

Experimental and therapeutic medicine

Yu D,Feng Y,Jiang Z,Yan T,Fang K,Shi Y,Zhang J,Zhang S

MA1-167 was used in RNA Immunoprecipitation to study the key role of HuR in modulating proliferation, senescence and radiosensitivity of skin cells providing a new therapeutic strategy for cosmetic treatments and to combat skin injury.

Thu Sep 01 00:00:00 UTC 2022

Long Noncoding RNA SCIRT Promotes HUVEC Angiogenesis via Stabilizing VEGFA mRNA Induced by Hypoxia.

Oxidative medicine and cellular longevity

Gao L,Yang J,Li Y,Liu K,Sun H,Tang J,Xia Z,Zhang L,Hu Z

MA1-167 was used in Western Blotting to reveal that lncRNA SCIRT can mediate endothelial angiogenesis by stabilizing the VEGFA mRNA via modulating RBP HuR stability under the HR condition.

Wed Jun 15 00:00:00 UTC 2022

Heat stress induced arginylation of HuR promotes alternative polyadenylation of Hsp70.3 by regulating HuR stability and RNA binding.

Cell death and differentiation

Deka K,Saha S

MA1-167 was used in Western Blotting to identify human antigen R (HuR) as a direct target of arginylation.

Mon Feb 01 00:00:00 UTC 2021

RNA-binding protein HuR regulates translation of vitamin D receptor modulating rapid epithelial restitution after wounding.

American journal of physiology. Cell physiology

Zhang Y,Cai JZ,Xiao L,Chung HK,Ma XX,Chen LL,Rao JN,Wang JY

MA1-167 was used in Immunocytochemistry to indicate that HuR enhances VDR translation by directly interacting with its mRNA via 3'-UTR and that induced VDR by HuR is crucial for rapid intestinal epithelial restitution after wounding.

Wed Jul 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like 1; Elav-like generic protein; ELAV-like protein 1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; HU-antigen A; Hu-antigen R; Human antigen R; HuR; MelG

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Gene Aliases: 2410055N02Rik; ELAV1; ELAVL1; Elra; Hua; HUR; MelG; W91709

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UniProt ID: (Human) Q15717, (Mouse) P70372, (Rat) B5DF91

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Entrez Gene ID: (Human) 1994, (Mouse) 15568, (Rat) 363854

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Function(s)

nucleotide binding RNA binding double-stranded RNA binding mRNA binding mRNA 3'-UTR binding protein binding AU-rich element binding protein kinase binding mRNA 3'-UTR AU-rich region binding protein homodimerization activity poly(A) RNA binding nucleic acid binding translation initiation factor

Process(es)

mRNA splicing, via spliceosome multicellular organism development regulation of mRNA stability positive regulation of translation mRNA stabilization negative regulation of gene silencing by miRNA 3'-UTR-mediated mRNA stabilization regulation of stem cell population maintenance regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HuR Monoclonal Antibody (3A2)

HuR Antibody (MA1-167) in ICC/IF

Product Details

MA1-167

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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