RP-10917 contains recombinant human BDNF protein suitable for use as a control in ELISA and Western blot applications.
RP-10917 is reported to have the additional biological activity: ED50 as determined by the dose-dependent induction of acetylcholine esterase in rat basal forebrain primary septal culture is 50 ng/mL.
Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long term storage add either HSA or BSA to 0.1%. Avoid repeat freeze/thaw cycles.
Reconstitute with 100 µg/mL of distilled water.
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Abrineurin; BDNF; Brain-derived Neurotrophic Factor; neurotrophin
Gene Aliases: ANON2; BDNF; BULN2
UniProt ID: (Human) P23560
Entrez Gene ID: (Human) 627