•Purity: ≥ 98% by SDS-Page and HPLC
•Endotoxin Concentration: <0.1 ng/µg
•Protein Length: 199 aa.
•Biological Activity: ED50 = 50-150 ng/mL
•Molecular Weight: 22.7 kDa
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute Ciliary Neurotrophic Factor in 5-10 mM sodium phosphate, pH 7.5, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
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Protein Aliases: Ciliary neurotrophic factor; CNTF; H-CNTF
Gene Aliases: CNTF; HCNTF
UniProt ID: (Human) P26441
Entrez Gene ID: (Human) 1270