Invitrogen

Human Furin Recombinant Protein

Product Details

RP-062

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 2 publications 2 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Control (Ctrl)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species	Human
Published species	Human
Expression System	Non-human primate
Amino acid sequence	Human Furin convertase protein, amino acids 1-713
Class	Recombinant
Type	Protein
Activity	Specific Activity = 1 U/µL; where 1 U is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	0.02M Bis-tris with 1mg/mL BSA
Contains	no preservative
Storage conditions	-80° C, Avoid Freeze/Thaw Cycles

Product Specific Information

RP-062 has an activity of 1 U/ul, where 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.

As a fully functional enzyme, RP-062 can also be used in a variety of enzymatic assays. 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in one minute (1 pmol of AMC/min).

This furin construct represents amino acid residues 1-713 from human furin convertase protein.

Target Information

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.