Description: Human IL-1 alpha, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), and mononuclear cell factor (MCF), is produced by a wide variety of cells, including macrophages, dendritic cells, T cells, and B cells. IL-1 alpha is mostly cell-associated and has only 20% amino acid homology with IL-1 beta. The immune regulatory role of IL-1 alpha is exerted on a wide range of cells including lymphocytes, epithelial cells, and fibroblasts. This cytokine induces hypotension, fever, and acute phase response in vivo.
Applications Reported: Recombinant human IL-1 alpha is biologically active and can promote proliferation of mouse D10S cells in culture.
Applications Tested: The ED50 of this protein, as measured by proliferation of mouse D10S cells, is less than or equal to 0.001 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e9 Units/mg.
Source: E. coli (amino acids Ser113-Ala271; accession # NP_000566).
Bioactivity: The ED50 of this protein, as measured by proliferation of mouse D10S cells, is less than or equal to 0.001 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e9 Units/mg.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.
Molecular Weight: 18 kDa.
Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex.Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hematopoietin-1; IL-1 alpha; interleukin 1, alpha; Interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552