RIL1025 contains 160 amino acid residues.
Purity: > 98% pure by SDS-PAGE and FPLC analyses.
Activity: One unit of activity is 2 ng/mL, as determined in a bioassay using the dose-dependent co-stimulation of MC/9 cells with mouse IL-4, corresponding to a specific activity of >5x10^5 units/mg.
Use: IL-10 exerts activity in the range of 0.2 to 20 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Reconstituted undiluted cytokine should be stored in working aliquots at -70°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586