•Purity: >95% by SDS-PAGE
•Endotoxin Concentration: <0.1 ng/µg Activity: ED50 = 2-6 ng/mL
•Molecular Weight: 12.5 kDa
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized hIL-13 should be reconstituted in sterile, deionized water to a concentration of 0.1-1.0 mg/mL to regain full activity. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: Lyophilized hIL-13 should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted hIL-13 at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596