Description: Human IL-7, also called lymphopoietin 1 (LP-1), a pre-B cell growth factor, is a ~28 kDa factor produced by bone marrow and thymic stromal cell and spleen cells. IL-7 is a stromal cell-derived growth factor for progenitor B cells and T cells. The main population in the thymus responsive to IL-7 is CD4^-CD8^-. It also stimulates proliferation and differentiation of mature T cells and NK cells.
Applications Reported: Recombinant human IL-7 is biologically active.
Applications Tested: This recombinant human IL-7 has been tested in bioassay of dose-dependent proliferation of PHA-activated normal human peripheral blood cells, yielding an ED50 of approximately 2.0 ng/mL, corresponding to a specific activity of approximately 5x10e5 Units/mg.
Source: E. coli expressed amino acids Asp26-His177 of mature human IL-7 accession # NM_000880.
Bioactivity: Measured by dose-dependent proliferation of PHA-activated human PBMCs. The ED50 is typically 2.0 ng/mL, corresponding to a specific activity of approximately 5x10e5 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The methionylated polypeptide has a predicted molecular mass of 17,518. The DTT reduced protein migrates as a 18 kDa polypeptide. The nonreduced protein migrates as a 16 kDa protein on non-reducing SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-7; Interleukin-7
Gene Aliases: IL-7; IL7
UniProt ID: (Human) P13232
Entrez Gene ID: (Human) 3574