|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IgA isolated from a pool of normal human sera|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:1000|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16388 targets Human IgA (alpha-Heavy Chain) in IHC (P), WB and FACS applications and shows reactivity with Human samples. This antibody is not recommended for Daudi cells in FACS applications. This antibody detects human and mouse IgA in Western blot.
The PA5-16388 immunogen is igA isolated from a pool of normal human sera.
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.