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Product Details
DIA HE1-01
Applications
Tested Dilution
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Human
/ IgE, kappa
Class
Monoclonal
Type
Isotype Control
Clone
HE1
Immunogen
monoclonal hybridoma from a healthy donor
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein L
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1075924
Product Specific Information
DIA HE1-01 contains purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B, but must be handled as potentially infectious, as should all human material.
The purity of this human IgE is >98%, tested by SDS-PAGE. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin E; ImmunoglobulinE
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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