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|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS with proprietary stabilizer|
|Storage Conditions||4° C, do not freeze|
|Cross Adsorption||Against goat IgG Fc, mouse, rat, bovine and horse serum proteins|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-33290 targets Human IgG in ELISA and IHC (F) applications. This antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis.
F(ab')2 fragments were prepared by pepsin digestion.
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.