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|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.8, with 3mg/ml BSA|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:20,000 - 1:400,000|
|Flow Cytometry (Flow)||1:200 - 1:1,000|
|Immunocytochemistry (ICC)||1:500 - 1:5,000|
|Immunofluorescence (IF)||1:500 - 1:5,000|
|Immunohistochemistry (IHC)||1:500 - 1:5,000|
|Immunoprecipitation (IP)||1:500 - 1:5,000|
|Western Blot (WB)||1:20,000 - 1:400,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 31770 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31770 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassy, this antibody cross-reacts with Rhesus monkey IgG (25%), swine IgM (12%), dog IgG, dog IgM, guinea pig IgG, horse IgG, human IgM, and swine IgG (6%), cat IgG, chicken IgG, hamster IgG and rabbit IgG (3%) and it has minimal cross-reactivity with immunoglobulins from other species.
Store product at 4°C. For long term storage, aliquots may be stored frozen.
Reconstitute with 1.0 ml of distilled water (1.5 mg/ml after restoration).
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.