|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse, rabbit and bovine serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Super-complexes of adhesion GPCRs and neural guidance receptors.
A-11014 was used in immunocytochemistry to analyze neural guidance receptors and super-complexes of adhesion GPCRs
|Jackson VA,Mehmood S,Chavent M,Roversi P,Carrasquero M,Del Toro D,Seyit-Bremer G,Ranaivoson FM,Comoletti D,Sansom MS,Robinson CV,Klein R,Seiradake E||Nature communications (7:null)||2016|
|Not Applicable||Not Cited||MLL5 maintains genomic integrity by regulating the stability of the chromosomal passenger complex through a functional interaction with Borealin.||Liu J,Cheng F,Deng LW||Journal of cell science (125:4676)||2012|
|Not Applicable||Not Cited||Bidirectional virus secretion and nonciliated cell tropism following Andes virus infection of primary airway epithelial cell cultures.||Rowe RK,Pekosz A||Journal of virology (80:1087)||2006|
|Not Applicable||Not Cited||GRIP domain-mediated targeting of two new coiled-coil proteins, GCC88 and GCC185, to subcompartments of the trans-Golgi network.||Luke MR,Kjer-Nielsen L,Brown DL,Stow JL,Gleeson PA||The Journal of biological chemistry (278:4216)||2003|
|Not Applicable||Not Cited||TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2.||Hayes S,Chawla A,Corvera S||The Journal of cell biology (158:1239)||2002|