|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.8, with 3mg/ml BSA|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:20,000 - 1:400,000|
|Flow Cytometry (Flow)||1:200 - 1:1,000|
|Immunocytochemistry (ICC)||1:500 - 1:5,000|
|Immunofluorescence (IF)||1:500 - 1:5,000|
|Immunohistochemistry (IHC)||1:500 - 1:5,000|
|Immunoprecipitation (IP)||1:500 - 1:5,000|
|Western Blot (WB)||1:20,000 - 1:400,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 31780 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31780 reacts with the kappa light chains of human immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody has minimal cross-reactivity with human lambda IgG (1%).
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 1.0 ml of distilled water (0.5 mg/ml after restoration).
The Thermo Scientific Secondary Antibodies featured here are affinity-purified polyclonal antibodies with well-characterized specificity for human immunoglobulin classes (IgG, IgM, IgA). Each of these anti-human immunoglobulin polyclonal antibodies is affinity-purified using its target antigen (i.e., the target human primary antibody class, subclass and fragment) coupled to an agarose support. Affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background. The purification process involves an elution procedure, that yields antibodies with high avidity. Conjugated antibodies are affinity-purified before being labeled and contain bovine serum albumin as a stabilizer. Species Abbreviation KeySelected secondary antibodies have been further purified to minimize cross-reactivity to serum proteins of other species. This purification is accomplished by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins of selected species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.