|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||human IgG whole molecule|
|Storage buffer||0.02M potassium phosphate, pH 7.4, with 10mg/ml BSA, 0.15M NaCl|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:6000 - 36000|
|Immunohistochemistry (Frozen) (IHC (F))||1:6000 - 36000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:6000 - 36000|
|Western Blot (WB)||1:6000 - 36000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28652 detects Human IgG from human samples.
PA1-28652 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot applications.
The PA1-28652 immunogen is: human IgG whole molecule
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.