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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified human IgM Fc 5 mu fragment.|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (IHC)||1:50 - 1:100|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SA1-400 reacts with human IgM, and does not react with human IgG, IgA, kappa or lambda chains.
SA1-400 has been successfully used in immunohistochemistry, ELISA, and Western blot procedures. Avoid prolonged fixation in buffered formalin as this can destroy the epitope.
The SA1-400 immunogen is purified human IgM Fc 5 mu fragment.
Country of Origin: USA
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.