Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Human Macrophage Colony Stimulating Factor (M-CSF, also called CSF-1) is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages.
Applications Reported: SAFE™ Human M-CSF Recombinant Animal Free Protein is biologically avtive.
Applications Tested: The ED50 of this protein, as measured by proliferation of M-NFS-60 cells, is < 2 ng/mL. This corresponds to a specific activity of > 5 x 10e5 Units/mg.
Source: Plant expressed 159 amino acids (Glu33-Ser190) with 16 amino acid Histidine tag. accession NP_757350.
Bioactivity: The ED50 of this protein, as measured by proliferation of M-NFS-60 cells, is <2 ng/mL. This corresponds to a specific activity of > 5 x 10e5 Units/mg.
Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE.
Molecular Weight: 20.7 kDa predicted, but migrates at apparent 24-26 kDa.
Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in deionized reagent grade water to a concentration no less than 100 µg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: colony stimulating factor 1 (macrophage); CSF-1; Lanimostim; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1
Gene Aliases: CSF-1; CSF1; MCSF
UniProt ID: (Human) P09603
Entrez Gene ID: (Human) 1435