|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Delipidated human milk fat globule membrane preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-13553 targets Human Milk Fat Globule 1 in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-13553 immunogen is delipidated human milk fat globule membrane preparation.
Human milk fat globule membrane protein (HMFG1) is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG1 is considered as a differentiation marker. It is useful as specific breast epithelial marker in normal and neoplastic mammary development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p63(TP63) elicits strong trans-activation of the MFG-E8/lactadherin/BA46 gene through interactions between the TA and DeltaN isoforms.
MA5-13553 was used in western blot to examine the role of p63 in the activation of MFG-E8/lactadherin/BA46 gene
|Okuyama T,Kurata S,Tomimori Y,Fukunishi N,Sato S,Osada M,Tsukinoki K,Jin HF,Yamashita A,Ito M,Kobayashi S,Hata RI,Ikawa Y,Katoh I||Oncogene (27:308)||2008|