|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full size recombinant E7 protein of HPV type 16|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23072 detects Papillomavirus Type 16 E7 Protein in Virus samples.
MA1-23072 has been successfully used in ELISA and Western Blot procedures. Predicted molecular weight of 11 kDA.
MA1-23072 immunogen corresponds to Full size recombinant E7 protein of HPV type 16.
The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.