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|Tested species reactivity||Virus|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bacterially derived fusion protein containing the human papillomavirus tp 16 early protein E7 open reading frame.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot against the HPV16-E7 bacterially derived fusion protein, the antibody detects a 52 kD band which is composed of the HPV16-E7 protein (15 kDa) and the trpE protein (37 kDa). Positive reactivity for the 15 kDa HPV16-E7 expressed protein was shown with CaSki (HPV16 DNA-containing cell line) cells by Western blot. The antibody has also been shown to immunoprecipitate and immunocytochemically stain the HPV16-E7 expressed protein from the CaSki cell line. Negative reactivity by Western blot was shown with a series of other HPV fusion proteins including: HPV16-E2, HPV16-E4, HPV16-E6, HPV18-E7, HPV6-E7, HPV6-E4, and HPV6-L1. The antibody failed to react with HeLa (HPV18 DNA-containing cell line) cells and other HPV DNA negative cervical carcinoma cell lines, HT-3 and C-33A, by Western blot, by immunoprecipitation or by immunocytochemical staining analyses.
Reconstitute the lyophilized material with 1 mL of deionized or distilled water for a final concentration of 50 µg/mL.
Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. E7-driven degradation of pRb may be involved in cervical tumorigenesis in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c).
28-0006 was used in western blot to study the interaction of human papillomaviruse E7 protein with B-Myb-MuvB complex and its effect on gene expression
|Pang CL,Toh SY,He P,Teissier S,Ben Khalifa Y,Xue Y,Thierry F||Oncogene (33:4039)||2014|
|Human||Not Cited||Reconstruction of human papillomavirus type 16-mediated early-stage neoplasia implicates E6/E7 deregulation and the loss of contact inhibition in neoplastic progression.||Isaacson Wechsler E,Wang Q,Roberts I,Pagliarulo E,Jackson D,Untersperger C,Coleman N,Griffin H,Doorbar J||Journal of virology (86:6358)||2012|
|Mouse||1:150||Two distinct activities contribute to human papillomavirus 16 E6's oncogenic potential.||Simonson SJ,Difilippantonio MJ,Lambert PF||Cancer research (65:8266)||2005|
|Cyclin G1 has growth inhibitory activity linked to the ARF-Mdm2-p53 and pRb tumor suppressor pathways.||Zhao L,Samuels T,Winckler S,Korgaonkar C,Tompkins V,Horne MC,Quelle DE||Molecular cancer research : MCR (1:195)||2003|
Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF.
28-0006 was used in immunoprecipitation to study the interaction between p14ARF and the human papillomavirus oncoprotein E7
|Dichamp I,Séité P,Agius G,Barbarin A,Beby-Defaux A||PloS one (9:null)||2014|
||Reconstruction of human papillomavirus type 16-mediated early-stage neoplasia implicates E6/E7 deregulation and the loss of contact inhibition in neoplastic progression.||Isaacson Wechsler E,Wang Q,Roberts I,Pagliarulo E,Jackson D,Untersperger C,Coleman N,Griffin H,Doorbar J||Journal of virology (86:6358)||2012|
|Human||Not Cited||The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini.||Debnath J,Mills KR,Collins NL,Reginato MJ,Muthuswamy SK,Brugge JS||Cell (111:29)||2002|