Surface Plasmon Resonance was performed to study the interaction between a Human Serum Albumin (HSA) monoclonal antibody (Product # MIH3005) and HSA. MIH3005 was covalently coupled to the surface of a planar mixed Self-Assembled Monolayer (SAM) slide via amine coupling using EDC-NHS chemistry (primary amines on the antibody were covalently attached to carboxyl groups on the surface of a gold slide containing a SAM (PEG spacer, 90% alcohol, and 10% carboxyl terminating groups). Different concentrations of HSA (80, 40, 20, 10, 5, 2.5, 1.25, 0.625, and 0.3125nM) were injected over the surface of the slide and micro Refractive Index Units (uRIU) were measured over time. Association and dissociation curves were determined using the global fitting software program, Scrubber™. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Serum Albumin|
|Storage buffer||0.015M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Competition Assay (CA)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MIH3005 targets Human Serum Albumin in ELISA, IP, and RIA applications and shows reactivity with Human samples.
The MIH3005 immunogen is human Serum Albumin.
MIH3005 detects Human Serum Albumin which has a predicted molecular weight of approximately 67 kDa.
MIH3005 was formerly sold as a Seradyn product.
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.