Recombinant human VEGF 165 is a non-glycosylated homodimer, containing two 165 amino acids, with a total molecular weight of 38.2 kDa. Purity is typically greater than 95% and endotoxin level less than 0.1 EU/ug.
The expected biological activity (ED50) is determined by the dose-dependent proliferation of HUVECs and is typically 1-6 ng/mL. The specific activity of this protein is 1x10^6 units/mg.
Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.
Amino acid sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H-VEGF-165; Vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF-A; VPF
Gene Aliases: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) Q6P0P5
Entrez Gene ID: (Human) 7422