This human recombinant protein was produced in E. coli.
Purity is greater than 95%.
RP-825 can be used as a positive control in Western blots with anti-p53 antibodies, as well as in ELISA and inhibition applications.
RP-825 calculated molecular weight is ~53 kDa.
This protein is stable at 4°C during shipment and for short term storage of 2-3 weeks. For long term storage, the protein should be kept frozen at -20°C or -80°C.
p53 is a tumor-suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. The p53 protein (393 aa, phosphoprotein) is a transcription factor, whose activity is regulated by phos-phorylation. The normal function of p53 is to effect cell cycle arrest at the G1- and G2-checkpoints in response to DNA damage. The current model postulates that p53 senses DNA damage and arrests the cell cycle to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death. In normal cells the p53 level is low. In response to DNA damage or other cellular stress signals the checkpoint function is executed by accumulation of p53. The control function of p53 includes the transcriptional regulation of several genes including the cell cycle inhibitor p21 WAF1/CIP1/SDI1 , DNA repair gene GADD45 and the apoptotic inducer Bax. p53 has also been shown to induce apoptosis by means of a direct signaling pathway. p53 directly binds to and acts on several cellular proteins involved in various pathways, including c-Abl or basal transcription factors. p53 can be functionally inactivated by mutation, most of them being amino acid substitutions. Inactivation can also be the result of binding to DNA tumor virus encoded proteins such as SV40 large T antigen, adenovirus E1B- and papilloma Virus E6-proteins or as a consequence of its interaction with the oncogene-encoded protein MDM2. The p53 tumor suppressor gene has been found to contain mutation in over 50% of human cancers. Many mutations of the p53 gene have been associated with malignant transformation in a wide variety of human tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.