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Immunofluorescent analysis of Hydrogen/Potassium ATPase beta using Anti-Hydrogen/Potassium ATPase beta Monoclonal Antibody (2G11) (Product# MA3-923) shows staining in A2058 Cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta (Product# MA3-923) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Dog, Human, Mustelid, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Rabbit, Rat, Rodent, Bovine, Mouse, Human, Guinea pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay dependent|
|Flow Cytometry (Flow)||1/50|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:2,000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-923 detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues.
MA3-923 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells.
MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site.
The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.
The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The use of murine-derived fundic organoids in studies of gastric physiology.
MA3-923 was used in flow cytometry and immunohistochemistry to evaluate an in vitro murine-derived fundic organoids for the study of gastric physiology
|Schumacher MA,Aihara E,Feng R,Engevik A,Shroyer NF,Ottemann KM,Worrell RT,Montrose MH,Shivdasani RA,Zavros Y||The Journal of physiology (593:1809)||2015|
Helicobacter pylori-induced Sonic Hedgehog expression is regulated by NFκB pathway activation: the use of a novel in vitro model to study epithelial response to infection.
MA3-923 was used in immunocytochemistry and immunohistochemistry - paraffin section to test if NFkappaB mediates H. pylori-induced sonic hedgehog
|Schumacher MA,Feng R,Aihara E,Engevik AC,Montrose MH,Ottemann KM,Zavros Y||Helicobacter (20:19)||2015|
Myosin IIB and F-actin control apical vacuolar morphology and histamine-induced trafficking of H-K-ATPase-containing tubulovesicles in gastric parietal cells.
MA3-923 was used in immunocytochemistry to study histamine-induced trafficking of gastric parietal cell H(+)/K(+)-ATPase-containing tubulovesicles and the modulatory roles of myosin II B and F-actin
|Natarajan P,Crothers JM,Rosen JE,Nakada SL,Rakholia M,Okamoto CT,Forte JG,Machen TE||American journal of physiology. Gastrointestinal and liver physiology (306:G699)||2014|
A possible mechanism for ezrin to establish epithelial cell polarity.
MA3-923 was used in immunocytochemistry to characterize the role of Ezrin in cytoskeletal function
|Zhu L,Crothers J,Zhou R,Forte JG||American journal of physiology. Cell physiology (299:C431)||2010|
Cyclopamine inhibition of the sonic hedgehog pathway in the stomach requires concomitant acid inhibition.
MA3-923 was used in immunocytochemistry to study the mechanism for the effect of cyclopamine on the sonic hedgehog pathway in the stomach
|El-Zaatari M,Grabowska AM,McKenzie AJ,Powe DG,Scotting PJ,Watson SA||Regulatory peptides (146:131)||2008|
Myosin II is present in gastric parietal cells and required for lamellipodial dynamics associated with cell activation.
MA3-923 was used in immunocytochemistry to investigate the role of myosin II in regulating lamellipodial dynamics associated with parietal cell activation
|Zhou R,Watson C,Fu C,Yao X,Forte JG||American journal of physiology. Cell physiology (285:C662)||2003|
PACAP stimulates gastric acid secretion in the rat by inducing histamine release.
MA3-923 was used in immunocytochemistry to investigate the role of PACAP during gastric acid secretion in the rat by inducing histamine release
|Sandvik AK,Cui G,Bakke I,Munkvold B,Waldum HL||American journal of physiology. Gastrointestinal and liver physiology (281:G997)||2001|
Functionally distinct pools of actin in secretory cells.
MA3-923 was used in immunocytochemistry to investigate the importance of cytoskeleton in the secretion of HCl in stomach
|Ammar DA,Nguyen PN,Forte JG||American journal of physiology. Cell physiology (281:C407)||2001|
Gastrin has a specific proliferative effect on the rat enterochromaffin-like cell, but not on the parietal cell: a study by elutriation centrifugation.
MA3-923 was used in immunocytochemistry to study the differential effect of gastrin on rat enterochromaffin-like cells and parietal cells
|Bakke I,Qvigstad G,Brenna E,Sandvik AK,Waldum HL||Acta physiologica Scandinavica (169:29)||2000|
Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase.
MA3-923 was used in immunocytochemistry to investigate the role of RAB11a N124I for stimulatory recruitment of the hydrogen-potassium-ATPase in gastric parietal cells
|Duman JG,Tyagarajan K,Kolsi MS,Moore HP,Forte JG||The American journal of physiology (277:C361)||1999|
Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor.
MA3-923 was used in immunocytochemistry to study the role of receptor extracellular domains in protein trafficking
|Dintzis SM,Velculescu VE,Pfeffer SR||The Journal of biological chemistry (269:12159)||1994|
The gastrin receptor antagonist netazepide (YF476) prevents oxyntic mucosal inflammation induced by Helicobacter pylori infection in Mongolian gerbils.
MA3-923 was used in immunohistochemistry to use a gastrin receptor antagonist to study the role of gastrin in Helicobacter pylori-induced oxyntic mucosal inflammation
|Sørdal Ø,Waldum H,Nordrum IS,Boyce M,Bergh K,Munkvold B,Qvigstad G||Helicobacter (18:397)||2013|
Parietal cell activation by arborization of ECL cell cytoplasmic projections is likely the mechanism for histamine induced secretion of hydrochloric acid.
MA3-923 was used in immunohistochemistry to study the mechanism for the histamine-induced secretion of hydrochloric acid in parietal cells
|Gustafsson BI,Bakke I,Hauso Ø,Kidd M,Modlin IM,Fossmark R,Brenna E,Waldum HL||Scandinavian journal of gastroenterology (46:531)||2011|
Genetic dissection reveals unexpected influence of beta subunits on KCNQ1 K+ channel polarized trafficking in vivo.
MA3-923 was used in immunohistochemistry to investigate the intracellular polarized transport of potassium channel KCNQ1 in vivo and its regulation
|Roepke TK,King EC,Purtell K,Kanda VA,Lerner DJ,Abbott GW||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:727)||2011|
K-ras mutation targeted to gastric tissue progenitor cells results in chronic inflammation, an altered microenvironment, and progression to intraepithelial neoplasia.
MA3-923 was used in immunohistochemistry to investigate the K-ras expression in stomach epithelium in response to Helicobacter pylori infection in a K-ras mutated mouse strain
|Okumura T,Ericksen RE,Takaishi S,Wang SS,Dubeykovskiy Z,Shibata W,Betz KS,Muthupalani S,Rogers AB,Fox JG,Rustgi AK,Wang TC||Cancer research (70:8435)||2010|
Impaired gastric gland differentiation in Peutz-Jeghers syndrome.
MA3-923 was used in immunohistochemistry to investigate the status of gastric gland differentiation in mice with Lkb1 mutations
|Udd L,Katajisto P,Kyyrönen M,Ristimäki AP,Mäkelä TP||The American journal of pathology (176:2467)||2010|
ERRgamma regulates cardiac, gastric, and renal potassium homeostasis.
MA3-923 was used in immunohistochemistry to study the role of estrogen-related receptor gamma on cardiac, gastric, and renal potassium homeostasis
|Alaynick WA,Way JM,Wilson SA,Benson WG,Pei L,Downes M,Yu R,Jonker JW,Holt JA,Rajpal DK,Li H,Stuart J,McPherson R,Remlinger KS,Chang CY,McDonnell DP,Evans RM,Billin AN||Molecular endocrinology (Baltimore, Md.) (24:299)||2010|
Pioglitazone induced gastric acid secretion.
MA3-923 was used in immunohistochemistry to investigate the effect of pioglitazone on the production of gastric acid
|Rotte A,Mack AF,Bhandaru M,Kempe DS,Beier N,Scholz W,Dicks E,Pötzsch S,Kuhl D,Lang F||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (24:193)||2009|
Transcription factors GATA-4 and GATA-6 in normal and neoplastic human gastrointestinal mucosa.
MA3-923 was used in immunohistochemistry to compare the expression level of GATA-4, GATA-6, and Ihh in normal and pathological human gastrointestinal tissue
|Haveri H,Westerholm-Ormio M,Lindfors K,Mäki M,Savilahti E,Andersson LC,Heikinheimo M||BMC gastroenterology (8:null)||2008|
|Guinea pig||Not Cited||
In situ measurement of pH in the secreting canaliculus of the gastric parietal cell and adjacent structures.
MA3-923 was used in immunohistochemistry to measure the pH in the secreting canaliculus of gastric parietal cells
|Schreiber S,Garten D,Nguyen TH,Konradt M,Bücker R,Scheid P||Cell and tissue research (329:313)||2007|
The KCNE2 potassium channel ancillary subunit is essential for gastric acid secretion.
MA3-923 was used in immunohistochemistry to demonstrate the essential role of KCNE2 for gastric acid secretion.
|Roepke TK,Anantharam A,Kirchhoff P,Busque SM,Young JB,Geibel JP,Lerner DJ,Abbott GW||The Journal of biological chemistry (281:23740)||2006|
Signet ring cells in gastric carcinomas are derived from neuroendocrine cells.
MA3-923 was used in immunohistochemistry to demonstrate that signet ring cells in gastric carcinomas originate from neuroendocrine cells
|Bakkelund K,Fossmark R,Nordrum I,Waldum H||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (54:615)||2006|
COUP-TFII is essential for radial and anteroposterior patterning of the stomach.
MA3-923 was used in immunohistochemistry to investigate COUP-TFII's functions during the formation of radial and anteroposteior patterning of the stomach
|Takamoto N,You LR,Moses K,Chiang C,Zimmer WE,Schwartz RJ,DeMayo FJ,Tsai MJ,Tsai SY||Development (Cambridge, England) (132:2179)||2005|
Overexpression of glycine-extended gastrin inhibits parietal cell loss and atrophy in the mouse stomach.
MA3-923 was used in immunohistochemistry to study the effect of glycine-extended gastrin overexpression on parietal cell loss and atrophy in the mouse stomach.
|Cui G,Koh TJ,Chen D,Zhao CM,Takaishi S,Dockray GJ,Varro A,Rogers AB,Fox JG,Wang TC||Cancer research (64:8160)||2004|
Role of adenosine A1 receptor in the regulation of gastrin release.
MA3-923 was used in immunohistochemistry to study the effect of adenosine A1 receptor on gastrin release
|Yip L,Leung HC,Kwok YN||The Journal of pharmacology and experimental therapeutics (310:477)||2004|
Neuroendocrine tumor markers and enterochromaffin-like cell hyper/dysplasia in type 1 diabetes.
MA3-923 was used in immunohistochemistry to investigate the association of CgA levels with enterochromaffin-like cell hyper/dysplasia in type 1 diabetes
|De Block CE,Colpin G,Thielemans K,Coopmans W,Bogers JJ,Pelckmans PA,Van Marck EA,Van Hoof V,Martin M,De Leeuw IH,Bouillon R,Van Gaal LF||Diabetes care (27:1387)||2004|
ECL-cell derived gastric cancer in male cotton rats dosed with the H2-blocker loxtidine.
MA3-923 was used in immunohistochemistry to study the induction of enterochromaffin-like cell-derived gastric cancer by pharmacological acid inhibition.
|Fossmark R,Martinsen TC,Bakkelund KE,Kawase S,Waldum HL||Cancer research (64:3687)||2004|
Role of adenosine A2A receptor in the regulation of gastric somatostatin release.
MA3-923 was used in immunohistochemistry to demonstrate that adenosine may act on A2A receptors to regulate gastric somatostatin release
|Yip L,Kwok YN||The Journal of pharmacology and experimental therapeutics (309:804)||2004|
High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.
MA3-923 was used in immunohistochemistry to show the subcellular localization of hydrogen/potassium-ATPase in gastric parietal cells
|Sawaguchi A,McDonald KL,Forte JG||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:77)||2004|
Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands.
MA3-923 was used in immunohistochemistry to investigate the distribution of NHE-3 in rat parietal cells and its functional activity
|Kirchhoff P,Wagner CA,Gaetzschmann F,Radebold K,Geibel JP||American journal of physiology. Gastrointestinal and liver physiology (285:G1242)||2003|
Autoimmune gastropathy in type 1 diabetic patients with parietal cell antibodies: histological and clinical findings.
MA3-923 was used in immunohistochemistry to study the histological and clinical characteristics of type 1 diabetic patients with parietal cell antibodies
|De Block CE,De Leeuw IH,Bogers JJ,Pelckmans PA,Ieven MM,Van Marck EA,Van Acker KL,Van Gaal LF||Diabetes care (26:82)||2003|
Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract.
MA3-923 was used in immunohistochemistry to study the role of Shh in gastric epithelial differentiation in adults.
|van den Brink GR,Hardwick JC,Nielsen C,Xu C,ten Kate FJ,Glickman J,van Deventer SJ,Roberts DJ,Peppelenbosch MP||Gut (51:628)||2002|
Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization.
MA3-923 was used in immunohistochemistry to study the biochemical characterization and subcellular localization of the clathrin in gastric acid secretory cells
|Okamoto CT,Duman JG,Tyagarajan K,McDonald KL,Jeng YY,McKinney J,Forte TM,Forte JG||American journal of physiology. Cell physiology (279:C833)||2000|
Octreotide inhibits the enterochromaffin-like cell but not peroxisome proliferator-induced hypergastrinemia.
MA3-923 was used in immunohistochemistry to investigate the effect of the octreotide on enterochromaffin-like cells
|Bakke I,Sandvik AK,Waldum HL||Journal of molecular endocrinology (25:109)||2000|
Modulatory role of phosphoinositide 3-kinase in gastric acid secretion.
MA3-923 was used in western blot to investigate the role of phosphoinositide 3-kinase in gastric acid secretion
|Mettler SE,Ghayouri S,Christensen GP,Forte JG||American journal of physiology. Gastrointestinal and liver physiology (293:G532)||2007|
Catalytic function of nongastric H,K-ATPase expressed in Sf-21 insect cells.
MA3-923 was used in western blot to investigate the function of nongastric hydrogen/potassium-ATPase expressed in Sf-21 insect cells
|Adams G,Tillekeratne M,Yu C,Pestov NB,Modyanov NN||Biochemistry (40:5765)||2001|
Identification of the beta-subunit for nongastric H-K-ATPase in rat anterior prostate.
MA3-923 was used in immunoprecipitation to study the subunit composition of nongastric H-K-ATPase in rat anterior prostate
|Pestov NB,Korneenko TV,Radkov R,Zhao H,Shakhparonov MI,Modyanov NN||American journal of physiology. Cell physiology (286:C1229)||2004|
ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR; ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide, gastric specific; gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric H+/K+-ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; gp60-90; H+,K+-ATPase; H+/K+ ATPase beta; H+/K+-ATPase beta; H,K-ATPase-Beta; H.K-ATPase, beta subunit; potassium-transporting ATPase beta chain; potassium-transporting ATPase subunit beta; proton pump beta chain
ATP4B; ATP6B; AV080843; BOS_12738; S76401S1