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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgM|
|Immunogen||Mouse MHC class II I-Ab|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17756 targets I-Ab in FACS applications and shows reactivity with Mouse samples.
The MA5-17756 immunogen is mouse MHC class II I-Ab.
The monoclonal antibody ER-TR3 reacts with mouse major histocompatibility complex class II (MHC class II), also know as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling.
MA5-17756 was used in flow cytometry to compare pro-Th2 DCs to pro-Th1 DCs
|Ferret-Bernard S,Castro-Borges W,Dowle AA,Sanin DE,Cook PC,Turner JD,MacDonald AS,Thomas JR,Mountford AP||Journal of proteomics (75:938)||2012|
|Not Applicable||Not Cited||
Dendritic cells activated with products released by schistosome larvae drive Th2-type immune responses, which can be inhibited by manipulation of CD40 costimulation.
MA5-17756 was used in flow cytometry to investigate how products released by schistosome larvae affect dendritic cells
|Jenkins SJ,Mountford AP||Infection and immunity (73:395)||2005|
Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.
MA5-17756 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.
|Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S||Clinical and experimental immunology (134:388)||2003|
H-2 class II histocompatibility antigen A beta chain; H-2 class II histocompatibility antigen, A beta chain; H2-AB1; H2-iabeta; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1
Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1