|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgM|
|Immunogen||Mouse MHC class II I-Ab|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-17757 targets I-Ab in FACS applications and shows reactivity with Mouse samples.
The MA5-17757 immunogen is mouse MHC class II I-Ab.
The monoclonal antibody ER-TR3 reacts with mouse major histocompatibility complex class II (MHC class II), also know as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
MA5-17757 was used in flow cytometry to identify key signals required for the exponential expansion of specific CD8 positive mouse T cells in vivo
|Wells JW,Cowled CJ,Farzaneh F,Noble A||Journal of immunology (Baltimore, Md. : 1950) (181:3422)||2008|