Invitrogen

I-Ab/I-Ad Monoclonal Antibody (25-9-17S), PE

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I-Ab/I-Ad Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Spleen cells stained using anti-I-Ab/I-Ad Monoclonal Antibody (Product # MA5-17777) at a 0.5 µg/10^6 cells dilution. The Isotype control was a PE Mouse IgG2a

Product Details

MA5-17777

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	25-9-17S
Immunogen	C3H.SW splenocytes
Conjugate	PE Biotin FITC
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.1 mg/mL
Purification	Protein G
Storage buffer	PBS with 4-5mg/mL BSA
Contains	0.02% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_2539161

Product Specific Information

MA5-17777 targets I-Ab/I-Ad in FACS applications and shows reactivity with Mouse samples.

The MA5-17777 immunogen is c3H.SW splenocytes.

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

UniProt ID: (Mouse) P14483

Entrez Gene ID: (Mouse) 14961

Molecular Function: major histocompatibility complex protein