Invitrogen

IBA1 Monoclonal Antibody (GT10312)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

13 Published Figures

14 References

View all (42) IBA1 antibodies

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Datasheet

Protocols

Questions & Answers

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IBA1 Antibody in Immunocytochemistry (ICC/IF) — IBA1 Monoclonal Antibody (GT10312) detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by IBA1 Monoclonal Antibody (GT10312) (Product # MA5-27726) diluted at 1:200. Blue: Hoechst 33342 staining.

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IBA1 Monoclonal Antibody (GT10312) detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 stained by IBA1 Monoclonal Antibody (GT10312) (Product # MA5-27726) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

IBA1 Antibody in Western Blot (WB) — Western blot was performed using Anti-IBA1 Monoclonal Antibody (GT10312) (Product # MA5-27726) and a 17 kDa band corresponding to IBA1 was observed in THP-1. Whole Cell Extract-WCL (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2), HeLa (Lane 3) and MCF7 (Lane 4), were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

IBA1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of TLR7 was performed in THP-1 cells using TLR7 Polyclonal Antibody (Product # PA1-28109) (red) at a dilution of 1:50. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

IBA1 Antibody in Immunohistochemistry (IHC) — Fig. 4 Immunohistochemical analysis of the AEA system. A Immunohistochemistry of naive male and female V1M cortex. Staining for NAPE-PLD, FAAH, Iba1, GFAP, and Nissl was performed. Original magnification x63. B Immunohistochemistry of naive male and female PAG. Staining for NAPE-PLD, FAAH, Iba1, GFAP, and Nissl was performed. Original magnification x63. C Measurement of relative area of IHC field occupied by NAPE-PLD staining pixels for each sex and region. Female NAPE-PLD immunoreactivity was significantly higher in PAG vs male PAG (* p = 0.02). D Measurement of co-efficient of colocalization for NAPE-PLD with IBa1, Nissl, and GFAP. NAPE-PLD colocalized with Nissl at significantly higher rates in female V1M cortex vs male V1M cortex (**** p = 0.001). No significant differences observed in PAG. E Measurement of relative area of IHC field occupied by FAAH staining pixels for each sex and region. FAAH immunoreactivity was significantly higher in female PAG vs male PAG (*** p = 0.0042) as well as female PAG vs female V1M cortex ( ### p = 0.002). F Measurement of co-efficient of colocalization for FAAH with IBa1, Nissl, and GFAP. No significant differences observed between region or sex. All calculations for co-efficient of colocalization were performed with respect to the relevant enzyme. n = 3/sex for each analysis

IBA1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Figure 3 PD-L1 ablation promoted Nep after SCI. (Ai, ii) Statistical analysis of the mechanical and thermal threshold in PD-LI WT and KO mice. (n = 5 mice per group at each time point, values are the mean +- SD, *p < 0.05, **p < 0.01, repeated measurement analysis of variance). (B, C) Western blot analysis of TRPV1 proteins in DRG (L4 and L5) of PD-L1 WT and KO mice post-SCI, and each lane corresponds to protein from an individual mouse (n = 6 mice per group at each time point, values are the mean +- SD, ns indicates no significance, **p < 0.01, ***p < 0.001, one-way ANOVA). (D, E) IF staining of the Neuron (red) and TRPV1 (green) in the DRG of the PD-L1 WT and KO mice at day 14 post-SCI. Nuclei were counterstained with DAPI (blue). (n = 6 mice per group, values are the mean +- SD, ***p < 0.001, two-tailed Student's t tests, scale bar = 100 mum). (F) Quantitative PCR of CCL2, TNF-alpha and IL-1beta in DRG of the PD-L1 WT and KO mice at day 14 post-SCI (n = 3 mice per group, values are the mean +- SD, *p < 0.05, **p < 0.01, two-tailed Student's t tests). (G, H) IF staining of the IBA-1 in the dorsal horn of PD-L1 WT and KO mice at day 14 post-SCI. Nuclei were counterstained with DAPI (blue) (n = 6 mice per group, values are the mean +- SD, **p < 0.01, two-tailed Student's t tests, scale bar = 100 mum).

IBA1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in IBA1 KO cell line compared to control cell line using Anti-IBA1 Monoclonal Antibody (GT10312)(Product # MA5-27726).

Product Details

MA5-27726

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT10312

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735228

Product Specific Information

Positive Control: THP-1, HL-60

Predicted Reactivity: Pig (93%), Rhesus Monkey (99%), Bovine (88%)

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microglial amyloid beta clearance is driven by PIEZO1 channels.

Journal of neuroinflammation

Jäntti H,Sitnikova V,Ishchenko Y,Shakirzyanova A,Giudice L,Ugidos IF,Gómez-Budia M,Korvenlaita N,Ohtonen S,Belaya I,Fazaludeen F,Mikhailov N,Gotkiewicz M,Ketola K,Lehtonen Š,Koistinaho J,Kanninen KM,Hernández D,Pébay A,Giugno R,Korhonen P,Giniatullin R,Malm T

MA5-27726 was used in Immunohistochemistry to indicate that the compromised function of microglia in AD could be improved by controlled activation of PIEZO1 channels resulting in alleviated Aβ burden.

Wed Jun 15 00:00:00 EDT 2022

Sevoflurane Aggravates the Progress of Alzheimer's Disease Through NLRP3/Caspase-1/Gasdermin D Pathway.

Frontiers in cell and developmental biology

Tian D,Xing Y,Gao W,Zhang H,Song Y,Tian Y,Dai Z

MA5-27726 was used in Immunohistochemistry, Immunocytochemistry to study the mechanisms of NLRP3/caspase-1 in pyroptosis and tau pathogenesis induced by sevoflurane.

Tue Feb 08 00:00:00 EST 2022

Sevoflurane Aggravates the Progress of Alzheimer's Disease Through NLRP3/Caspase-1/Gasdermin D Pathway.

Frontiers in cell and developmental biology

Tian D,Xing Y,Gao W,Zhang H,Song Y,Tian Y,Dai Z

MA5-27726 was used in Immunohistochemistry, Immunocytochemistry to study the mechanisms of NLRP3/caspase-1 in pyroptosis and tau pathogenesis induced by sevoflurane.

Tue Feb 08 00:00:00 EST 2022

Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids.

Cells

Fagerlund I,Dougalis A,Shakirzyanova A,Gómez-Budia M,Pelkonen A,Konttinen H,Ohtonen S,Fazaludeen MF,Koskuvi M,Kuusisto J,Hernández D,Pebay A,Koistinaho J,Rauramaa T,Lehtonen Š,Korhonen P,Malm T

MA5-27726 was used in Immunohistochemistry to conclude that microglia-like cells within the organoids promote neuronal and network maturation and recapitulate some aspects of microglia-neuron co-development in vivo, indicating that cerebral organoids could be a useful biorealistic human in vitro platform for studying microglia-neuron interactions.

Thu Dec 30 00:00:00 EST 2021

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

International journal of molecular sciences

Yu CG,Bondada V,Iqbal H,Moore KL,Gensel JC,Bondada S,Geddes JW

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Western Blot

Wed Dec 29 00:00:00 EST 2021

Sex differences in the expression of the endocannabinoid system within V1M cortex and PAG of Sprague Dawley rats.

Biology of sex differences

Levine A,Liktor-Busa E,Lipinski AA,Couture S,Balasubramanian S,Aicher SA,Langlais PR,Vanderah TW,Largent-Milnes TM

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry

Mon Nov 08 00:00:00 EST 2021

Sex differences in the expression of the endocannabinoid system within V1M cortex and PAG of Sprague Dawley rats.

Biology of sex differences

Levine A,Liktor-Busa E,Lipinski AA,Couture S,Balasubramanian S,Aicher SA,Langlais PR,Vanderah TW,Largent-Milnes TM

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry

Mon Nov 08 00:00:00 EST 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry (PFA fixed)

Mon Nov 01 00:00:00 EDT 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry (PFA fixed)

Mon Nov 01 00:00:00 EDT 2021

NLRP3 inhibition attenuates early brain injury and delayed cerebral vasospasm after subarachnoid hemorrhage.

Journal of neuroinflammation

Dodd WS,Noda I,Martinez M,Hosaka K,Hoh BL

MA5-27726 was used in Immunohistochemistry to observe a decrease in apoptosis of neurons in mice treated with MCC950.

Tue Jul 20 00:00:00 EDT 2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunocytochemistry

Sat May 22 00:00:00 EDT 2021

Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice.

Journal of neuroinflammation

Feng YK,Wu QL,Peng YW,Liang FY,You HJ,Feng YW,Li G,Li XJ,Liu SH,Li YC,Zhang Y,Pei Z

MA5-27726 was used in Immunohistochemistry-immunofluorescence to investigate the effect of the bacterium porphyromonas gingivalis (Pg) on the gut and brain when administered orally in mice.

Thu Nov 19 00:00:00 EST 2020

Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures.

Cells

Zhang I,Lépine P,Han C,Lacalle-Aurioles M,Chen CX,Haag R,Durcan TM,Maysinger D

MA5-27726 was used in Immunohistochemistry to study glia modulation by dendritic polyglycerol sulfate.

Sat Nov 07 00:00:00 EST 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

MA5-27726 was used in Immunohistochemistry to propose that osteopontin may act as a molecular brake within the brain in order to regulate the inflammatory response to chronic viral infections, such as HIV.

Thu Sep 17 00:00:00 EDT 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

Thu Sep 17 00:00:00 EDT 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

Thu Sep 17 00:00:00 EDT 2020

SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence.

Biomolecules

Merlo S,Luaces JP,Spampinato SF,Toro-Urrego N,Caruso GI,D'Amico F,Capani F,Sortino MA

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry

Thu Feb 27 00:00:00 EST 2020

SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence.

Biomolecules

Merlo S,Luaces JP,Spampinato SF,Toro-Urrego N,Caruso GI,D'Amico F,Capani F,Sortino MA

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunohistochemistry

Thu Feb 27 00:00:00 EST 2020

Bioinformatics

Protein Aliases: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

Molecular Function: calmodulin-related

Function(s)

Process(es)

molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding

IBA1 Monoclonal Antibody (GT10312)

Product Details

MA5-27726

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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